Knesset Speaker Dalia Itzik and former minister Natan Sharansky also spoke at the gathering, organized by a coalition of American Jewish groups.
Wiesel said "We urge all the UN delegations across the street to leave the hall when [Ahmadinejad] appears on the stage."
"Mahmoud Ahmadinejad, go home and stay there, we don't want you here. America doesn't want you here. Nobody wants you," Wiesel called out.
The event sparked further controversy over the weekend when Republican vice presidential hopeful Sarah Palin's appearance in the anti-Iran rally in Dag Hammarskjold Plaza was cancelled in a flap between protest organizers and Hillary Clinton, who had also been scheduled to speak.
Clinton aides were quoted as saying that they had been "blindsided" by the decision to invite Palin, which they called a partisan move. In the ensuing controversy, Clinton withdrew her participation, and Palin's invitation was rescinded.